Analysis Note

Positive ControlPC-12, HCT116, or HeLa cells treated with DNA damaging agents

Application

Immunoblotting (1:1000)Immunoprecipitation (1:200)

General description

Recognizes the ~53 kDa p53 protein phosphorylated at Ser¹⁵ in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.

This PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser¹⁵. r>

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.

Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.Levine, A.J. 1997. Cell88, 323.Milczarek, G.J., et al. 1997. Life Sci.60, 1.Shieh, S.Y., et al. 1997. Cell91, 325.Meek, D.W. 1994. Semin Cancer Biol.5, 203.

Packaging

50 µL in Plastic ampoule

Physical form

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Warning

Toxicity: Standard Handling (A)